Product Overview

Aniline Blue (Water Soluble) For Microscopy (CAS No. 28983-56-4) is a water-soluble biological dye used primarily for staining tissue components in microscopy. One of its established histological roles is the blue staining of collagen and related connective-tissue structures, providing clear visual contrast between these fibres and the surrounding cellular material.

The Microscopy grade is intended for the preparation of staining solutions used in histological and cytological work. It is particularly relevant to connective-tissue methods, including Mallory-type staining, and may also be used in Gomori one-step trichrome procedures when methyl blue is specified as the blue component. Its water solubility allows straightforward preparation of aqueous stain solutions, making it suitable for routine microscopy work where consistent colour development and clear tissue differentiation are required.

Typical Applications

• Staining of collagen in histological tissue sections
• Differentiation of connective-tissue structures during light microscopy
• Mallory connective-tissue staining procedures
• Gomori one-step trichrome staining where methyl blue is specified
• Preparation of aqueous staining solutions for microscopy
• Differential staining in histological and cytological preparations
• Teaching and research microscopy involving connective-tissue morphology

Common Synonyms

• Acid Blue 93
• Methyl Blue
• Cotton blue
• Poirriers blue
• Water blue
• C.I. 42780
• Methyl Blue Sodium Salt
• Aniline Blue, Water Soluble

Storage Conditions

Keep the material tightly closed in a dry place and protect it from moisture and prolonged exposure to light. Reseal the container promptly after dispensing and avoid contamination of the powder so that its solubility and staining behaviour remain consistent.