Product Overview

Benzene 99.8% For HPLC & UV Spectroscopy (CAS No. 71-43-2) is the parent monocyclic aromatic hydrocarbon, defined by a planar six-membered ring with a delocalised π-electron system. Its low polarity and uniform aromatic environment provide distinctive solvating characteristics for hydrophobic and weakly polar organic compounds. Benzene also exhibits intrinsic ultraviolet absorption, making solvent purity and selection of a compatible measurement wavelength important in optical applications.

The HPLC & UV Spectroscopy grade is intended for liquid-chromatographic and spectrophotometric procedures requiring control of extraneous ultraviolet-absorbing impurities, non-volatile residues, and particulate contamination. It is appropriate for normal-phase HPLC methods that specifically prescribe benzene as a mobile phase or mobile-phase component, for preparing compatible sample and standard solutions, and for UV measurements performed within spectral regions where benzene provides an acceptable solvent blank.

Typical Applications

• Mobile phase or mobile-phase component in normal-phase HPLC methods that specifically prescribe benzene.
• Preparation of sample, standard, and reference solutions for hydrophobic or weakly polar analytes in compatible chromatographic procedures.
• Solvent-selectivity, retention, and adsorption studies involving non-polar aromatic mobile phases.
• Solvent for UV-visible spectroscopic examination of compatible non-polar organic compounds at suitable measurement wavelengths.
• Preparation of matched solvent blanks and reference solutions for ultraviolet spectroscopic measurements.
• HPLC-UV method-development work in which solvent background, baseline behaviour, and non-volatile contamination require careful control.

Common Synonyms

• Benzol
• Benzole
• [6]Annulene
• Cyclohexa-1,3,5-triene
• 1,3,5-Cyclohexatriene

Storage Conditions

Store in a tightly closed container in a cool, dry, and well-ventilated area. Protect from direct sunlight, excessive heat, ignition sources, evaporation, and contamination. Minimise unnecessary container opening and use clean, solvent-compatible dispensing equipment to preserve chromatographic and spectroscopic cleanliness.