Product Overview

Silica Gel GF 254 For Thin Layer Chromatography (CAS No. 112926-00-8) is a silica-based TLC adsorbent combining the G binder designation with an F254 fluorescent indicator system. The silica surface provides polar normal-phase adsorption, while the binder supports adherence of coated layers to the plate. The F254 functionality enables visualisation under ultraviolet radiation near 254 nm when UV-absorbing sample zones reduce or quench the fluorescence of the background layer.

The Thin Layer Chromatography form is intended for preparation of bonded-to-plate silica layers with built-in short-wave UV visualisation capability. It supports rapid reaction monitoring, qualitative identity comparison and purity assessment without requiring every analyte to be chemically derivatised for detection.

Typical Applications

• Preparation of laboratory-coated fluorescent TLC plates.
• Visualisation of UV-absorbing compounds by fluorescence quenching at 254 nm.
• Monitoring organic reactions by TLC.
• Qualitative identity and purity comparison of suitable compounds.
• Selection of solvent systems for preparative chromatography.
• Fraction monitoring during column-chromatographic purification.

Common Synonyms

• Silica gel GF254
• TLC silica gel GF254
• Silica gel GF 254
• Thin-layer chromatography silica gel GF254

Storage Conditions

Keep tightly closed, dry and protected from contamination that could modify silica activity or introduce fluorescent/UV-absorbing background. Maintain clean storage and handling so that both the adsorptive surface and F254 visualisation response remain suitable for TLC use.