Product Overview

Silica Gel HF 254 For Thin Layer Chromatography (CAS No. 112926-00-8) is an H-type silica TLC adsorbent incorporating an F254 fluorescent indicator while retaining the binder-free functional character associated with the H designation. Its silanol-rich surface provides polar adsorption for normal-phase separation, and the fluorescent background enables detection of UV-absorbing compounds through quenching or attenuation when examined at approximately 254 nm.

The Thin Layer Chromatography form is intended for preparation of fluorescent TLC layers in procedures that specifically require H-type silica rather than conventional binder-containing G material. It is suitable for reaction monitoring, qualitative comparison and purity assessment of UV-active compounds.

Typical Applications

• Preparation of fluorescent binder-free TLC layers.
• Detection of UV-absorbing compounds by fluorescence quenching at 254 nm.
• Monitoring of organic synthesis reactions.
• Qualitative identity and purity assessment.
• Solvent-system optimisation before preparative chromatography.
• TLC procedures specifically requiring HF254-type silica.

Common Synonyms

• Silica gel HF254
• TLC silica gel HF254
• Silica gel HF 254
• Thin-layer chromatography silica gel HF254

Storage Conditions

Keep tightly closed and dry to preserve silica adsorption activity and coating reproducibility. Protect from fluorescent, UV-absorbing, oily or particulate contamination that could interfere with F254 background response or chromatographic separation.