Product Overview

Tris Buffer 99% Extra Pure (CAS No. 77-86-1) is tris(hydroxymethyl)aminomethane, a water-soluble organic amino alcohol containing one primary amino group and three hydroxymethyl groups. Commonly called Tris or Tris base, it behaves as a weak organic base and is widely used to prepare aqueous buffering systems, particularly in the near-neutral to mildly alkaline region. Its acid–base equilibrium is temperature-sensitive, so the pH of Tris-containing solutions can change appreciably when the temperature changes.

The Extra Pure grade is intended for routine laboratory buffer preparation, general reagent formulation and pH-control work where a dependable Tris buffer substance is required without the additional application-specific controls associated with analytical, chromatographic or molecular-biology grades. It can be combined with a suitable acid, commonly hydrochloric acid, to prepare Tris-based buffers over its effective buffering region and is useful in general laboratory procedures requiring stable control of moderately alkaline pH.

Typical Applications

• Preparation of routine Tris and Tris-HCl laboratory buffer solutions
• General pH control in aqueous laboratory reagent systems
• Preparation of buffer stocks for routine laboratory procedures
• Adjustment and maintenance of mildly alkaline solution conditions
• General biochemical and chemical laboratory buffer preparation
• Formulation of aqueous reagent systems requiring an organic amine buffer
• Laboratory studies involving buffer capacity and pH-dependent processes

Common Synonyms

• Tris
• Tris Base
• Tris(hydroxymethyl)aminomethane
• Trometamol
• Tromethamine
• THAM
• 2-Amino-2-(hydroxymethyl)propane-1,3-diol

Storage Conditions

Store tightly closed in a cool, dry place and protect from moisture and contamination. Reseal the container promptly after use to preserve the composition and quality of the dry buffer substance.