Product Overview

Tris Buffer 99.5% AR/HPLC (CAS No. 77-86-1) is tris(hydroxymethyl)aminomethane, a highly water-soluble amino alcohol that accepts protons through its primary amine and forms effective aqueous buffering systems in the near-neutral to mildly alkaline range. Its buffering equilibrium is strongly influenced by temperature, making control of preparation and measurement temperature relevant whenever reproducible buffer pH is required.

The AR/HPLC grade is intended for analytical reagent preparation and chromatographic buffer systems requiring controlled Tris quality. In conventional liquid chromatography, Tris is useful as an aqueous mobile-phase buffer in method-appropriate separations, particularly ion-exchange and biomolecule chromatography where control of pH influences analyte charge and retention. The grade is also suited to analytical buffer preparation, sample-treatment solutions and laboratory procedures in which reagent purity and reproducible buffer composition are important.

Typical Applications

• Preparation of Tris-based aqueous mobile phases for suitable HPLC methods
• Buffering of ion-exchange chromatographic separations
• Preparation of mobile phases for protein and nucleic-acid chromatography
• Analytical reagent and buffer preparation for pH-controlled laboratory procedures
• Preparation of sample-treatment and extraction buffers used in analytical workflows
• Method development involving pH-dependent chromatographic retention
• Routine analytical quality-control work requiring high-purity Tris buffer substance

Common Synonyms

• Tris
• Tris Base
• Tris(hydroxymethyl)aminomethane
• Trometamol
• Tromethamine
• THAM
• 2-Amino-2-(hydroxymethyl)propane-1,3-diol

Storage Conditions

Store tightly closed in a cool, dry place and protect from moisture and contamination. Maintain the reagent in its original well-sealed container and minimize prolonged exposure to humid laboratory air.