Product Overview

Tris Buffer 99.9% For Molecular Biology (CAS No. 77-86-1) is tris(hydroxymethyl)aminomethane, a water-soluble amino alcohol widely used as a buffering component in nucleic-acid, protein and enzyme-related laboratory systems. Its useful buffering region encompasses many near-neutral to mildly alkaline biochemical conditions, while its pronounced temperature dependence means that Tris-buffered solutions should be prepared and adjusted with consideration of the temperature at which they will be used.

The Molecular Biology grade is intended for preparation of high-purity Tris-based buffers used in DNA, RNA and protein workflows. Tris serves as a principal buffering component in electrophoresis buffers, nucleic-acid handling solutions, extraction and washing buffers, enzyme-compatible reagent systems and Tris-buffered saline formulations. Its role is to maintain controlled pH during biomolecular manipulations in which changes in proton concentration can influence nucleic-acid stability, protein behaviour and enzyme performance.

Typical Applications

• Preparation of Tris-HCl buffers for molecular-biology procedures
• Preparation of TAE and TBE electrophoresis buffer systems
• Buffering of DNA and RNA extraction, purification and handling solutions
• Preparation of Tris-buffered saline and related washing buffers
• Buffering of protein extraction and purification procedures
• Preparation of enzyme-reaction and biomolecular assay buffers
• General nucleic acid and protein laboratory workflows requiring controlled mildly alkaline pH

Common Synonyms

• Tris
• Tris Base
• Tris(hydroxymethyl)aminomethane
• Trometamol
• Tromethamine
• THAM
• 2-Amino-2-(hydroxymethyl)propane-1,3-diol

Storage Conditions

Store tightly closed in a cool, dry place and protect from moisture and contamination. Use clean dispensing practices and reseal promptly after use to preserve reagent quality for sensitive molecular biology buffer preparation.