Packings
500 ml
500 ml

Product Information

Basic information about this product

Article No. 007366
Grade Molecular Biology
Purity N/A
CAS No. 9005-64-5
Molecular Formula C58H114O26
Molecular Weight 1227.72
H.S. Code 34024200
Shelf Life 60 Months

Hazard Information

Safety and hazard classification

NO GHS NO GHS
Hazard Symbols
NO GHS

Safety Information

Important safety instructions and precautions

Signal Word: N/A
UN No.: N/A
IMCO Class No.: N/A
Packing Group: N/A
Hazardous Statement: N/A
Precaution Statement: N/A

Product Description

Detailed information about the product

Product Overview

Tween 20 For Molecular Biology (CAS No. 9005-64-5), commonly known as polysorbate 20, is a hydrophilic nonionic surfactant based on polyoxyethylene sorbitan laurate structures. Its amphiphilic character enables micelle formation and mild detergent action in aqueous systems, allowing interaction with hydrophobic membrane components and proteins while avoiding the ionic charge associated with anionic or cationic detergents.

The Molecular Biology grade is intended for biochemical and molecular-biology procedures in which Tween 20 functions as a controlled nonionic detergent component of aqueous buffers and reagent systems. It is particularly useful in membrane-protein solubilization and in washing, blocking and dilution buffers used in protein detection workflows, where reduction of nonspecific interactions and effective wetting of membrane surfaces are important. Its water compatibility also supports detergent-containing sample-preparation and biomolecular handling procedures in which mild surfactant action is required.

Typical Applications

  • Preparation of washing buffers for Western blotting and related membrane-based assays
  • Preparation of blocking and antibody-dilution buffer systems
  • Solubilization and handling of membrane-associated proteins
  • Mild detergent treatment during protein extraction and purification workflows
  • Reduction of nonspecific interactions in immunoblotting and immunoassay procedures
  • Preparation of detergent-containing buffers for molecular-biology and biochemical workflows
  • Wetting and uniform reagent distribution across membrane surfaces

Common Synonyms

  • Polysorbate 20
  • Polyoxyethylene (20) Sorbitan Monolaurate
  • Polyoxyethylene Sorbitan Monolaurate
  • Polyethylene Glycol Sorbitan Monolaurate
  • POE (20) Sorbitan Monolaurate

Storage Conditions

Store tightly closed in a cool, dry place and protect from prolonged exposure to light, heat and air. Polysorbate 20 is susceptible to gradual oxidative degradation and peroxide formation during storage, particularly under elevated-temperature conditions.